- Author
- Song J,Bishop BL,Li G,Grady R,Stapleton A,Abraham SN
- Title
- TLR4-mediated expulsion of bacteria from infected bladder epithelial cells.
- Publication Date
- 8/17/2009
- Pages
- -
- Journal Name
- Proceedings of the National Academy of Sciences of the United States of America
- Abstract
- Uropathogenic Escherichia coli invade bladder epithelial cells (BECs) by direct entry into specialized cAMP regulated exocytic compartments. Remarkably, a significant number of these intracellular bacteria are subsequently expelled in a nonlytic and piecemeal fashion by infected BECs. Here, we report that expulsion of intracellular E. coli by infected BECs is initiated by the pattern recognition receptor, Toll-like receptor (TLR)4, after activation by LPS. Also, we reveal that caveolin-1, Rab27b, PKA, and MyRIP are components of the exocytic compartment, and that they form a complex involved in the exocytosis of bacteria. This capacity of TLR4 to mediate the expulsion of intracellular bacteria from infected cells represents a previously unrecognized function for this innate immune receptor.
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